Assessment of Healing Potential of Formulated Herbal Cream on Burn Wound

The current protocol of the study was planned to assess the healing potential of formulated herbal cream containing different medicinal plant extracts. Formulated herbal cream was containing the methanolic extract of Chenopodium album, Coccinia indica, Momordica dioica, Precitrullus fistulosus and Trichosanthes dioica with cream base. Formulated herbal cream was assessed pharmacologically for healing potential in burn wound model. The formulation showed a significantly higher contraction rate, shortened epithelialization period, better tensile strength and increase in hydroxyproline content in the burn wound model. Thus, it is concluded that formulation has the potential wound healing activity which justifying its use as wound healer. Keywords:  Formulation, Burn, Wound, Contractio

Phytochemical Appraisal of Cucurbitaceae Plants Extracts

The present study deals with the phytochemical screening of selected medicinal plant drugs of Cucurbitaceae family. Leaves of test plants (Coccinia indica, Momordica dioica, Praecitrullus fistulosus and Trichosanthes dioica) were extracted successively with petroleum ether, methanol and water by hot extraction method and screened for the presence of alkaloids, carbohydrates, glycosides, flavonoids, proteins, resins, steroids and tannins. Obtained findings concluded that selected plants are good source of various phytochemicals. This experimental work revealed the presence of various biologically active secondary metabolites which could be helpful in the lead to drug discovery and development. Keywords: Phytochemical, Screening, Medicinal plants, Extrac

Formulation and Evaluation of Etoricoxib Herbal Gel for Analgesic Activity

Medicinal plants play an imperative function in the development of potent therapeutic agents. The existing study has been undertaken with the plan to formulate and evaluate the etoricoxib herbal gel containing Buchanania lanzan extract for the management and treatment of pain. The etoricoxib herbal gel formulation was intended by using etoricoxib, menthol, linseed oil with different polymers and additives in the composition of gel. Formulated gel was evaluated in terms of various evaluation parameters as physicochemical assessment, pH, viscosity and spreadibility. Further, formulated etoricoxib herbal gel was investigated for analgesic activity by using hot-plate and acetic acid induced writhing methods. The obtained findings conclude that formulated etoricoxib herbal gel is the appropriate option for the treatment of pain and such other indications. Keywords: Pain, Analgesic, Etoricoxib, Buchanania lanza

INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA

An in vitro study with Ri-TDNA transformed roots of carrot  (Daucus carota) was carried out  to evaluate  the role of macro-elements contributed as impurities in  the gelling agent (phytagel) over and above  those present  in the minimal (M) medium. Production of root biomass was taken as a measure to quantify the influence of macro-elements added to the minimal medium. The levels of phosphorus when adjusted to 1.19 mg/1 and 1.09 mg/l, lead to dry root biomass production at par with the control. Attempts made to lower the amount of impurities in phytagel by de-ionization using different alkalies, proved NaOH to give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum number of infection units and auxiliary cells when phytagel was added to the minimal medium.Key words:    Agrobacterium    rhiiogenesfDaucus    caro/a/Gelling    agents/diaspora    margarita/Macro- elements/Vesicular-arbuscular mycorrhiza/Transformed roots

Formulation and In-Vitro evaluation of Nanocrystal formulation of poorly soluble drugs

Introduction:-Poor solubility of drug compounds which accounts for 40% of new molecules investigated at present is an issue of great concern in pharmaceutical industry and reducing particle size (i,e to reduce below 1000 nm )of drug candidate to be investigated is one of the simplest and efficient ways to overcome this challenge. Drug nanocrystals, solid nanosized drug particles are defined as formulation having 100% drug, which are covered by a stabilizer layer. In this study attempt was made to formulate and evaluate nanocrystals of poorly soluble drugs having low oral bioavailability. Material and method:- Nanocrystals were prepared successfully by varying concentration of different stabilizers by anti-solvent precipitation method. The formulated nanocrystals were evaluated by determining physicochemical characteristics such as physical appearance, Differential Scanning Calorimetry (DSC), scanning electron microscopy (SEM), X-ray powder diffractometry, solubility studies, particle size distribution, zeta potential, and in vitro drug release profile studies. Results:- An in-vitro study was performed on the successful formulation in comparison to drug powder using dissolution apparatus The particle size of RVT and PSNC-3 was found to be 1975.3 nm and 790.1 nm respectively. Conclusion: Precipitated Nanocrystals formulated with different stablizer’s method resultedin formation of small and uniform RVT nanocrystals with an improved saturation solubility, dissolution rate. Keywords: Nanocrystal, poorly soluble drug

Formulation and Evaluation of Anti-inflammatory Gel

The current study has been undertaken with the aim to formulate and evaluate the etoricoxib gel containing Buchanania lanzan extract. The gel formulation was designed by using etoricoxib, menthol, linseed oil with different polymers in the composition of gel. Formulated gel was evaluated in terms of various physicochemical parameters, pH, viscosity and spreadibility. Further formulated gel was investigated for anti-inflammatory activity by using Carrageenan-induced rat paw edema method. The obtained findings state that formulated gel is the suitable choice for the treatment of inflammation and such other indications. Keywords: Inflammation, Gel, Herbal, Formulatio

Screening of Phytochemicals and Antioxidant Potential of Leaves Extract of Litsea glutinosa

Litsea glutinosa (Lour) leaves are deliberated as worthy traditional medicine. The aim of this study was to screen the phytochemicals, to evaluate the total flavonoid contents as well as antioxidant activity of various extract of Litsea glutinosa (Lour). The phytochemical analysis of each solvent extract was also carried out. Total flavonoid content was determined by aluminium chloride colorimetric assay. Antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing ability of plasma (FRAP) and Hydrogen Peroxide (H2O2) free radical scavenger methods. Phytochemical screening of various extract revealed the presence of Alkaloids, Flavonoids, Diterpenes, Proteins, Carbohydrate and Saponins. The total flavonoid content was found 1.89, 5.01 and 3.16 mg/100mg of dry weight of pet ether, ethanol and aqueous extract respectively, expressed as Quercetin equivalents. Antioxidant activity was performed using three methods DPPH, Ferric reducing ability of plasma (FRAP) assay and Hydrogen Peroxide scavenging activity.  Our current results emerged that Litsea glutinosa (Lour) act as an antioxidant agent due to its free radical scavenging. So, the plant may be further pursued to find out for its pharmacological active natural products. Keywords: Litsea glutinosa (Lour), Qualitative, Quantitative phytochemical, Antioxidant activity

Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Chloroform extract of Argemone mexicana

Aim: The present study was undertaken to examine the effects of Chloroform extract of Argemone mexicana using the paracetamol-induced liver damage in rats as the animal model. Materials and methods: Chloroform extract of Argemone mexicana (100 and 200 mg/kg body weight) was administered daily in experimental animals. The hepatoprotective efficacy of Chloroform extract of Argemone mexicana (100 and 200 mg/kg) was investigated against paracetamol-induced hepatotoxicity. The levels of serum glutamic oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), serum alkaline phosphatase (ALP), bilirubin and triglycerides were estimated. Moreover, chloroform extract of Argemone mexicana -aided antioxidant defense against hepatotoxic insult of paracetamol was measured by evaluating a number of anti-oxidative biomarkers including reduced malondialdehyde (MDA) in the serum. Results: Oral administration of paracetamol (500 mg/kg b.wt.) resulted in a significant elevation of liver enzymes in serum such as SGOT, SGPT, ALP, bilirubin and triglyceride levels when compared with the results in the control group. As regards oxidative stress biomarkers, there were increased tissue levels of malondialdehyde in the group treated with paracetamol. All of these results were ameliorated by co-administration of chloroform extract of Argemone mexicana. Conclusions: These results suggest that the protective role of Chloroform extract of Argemone mexicana in the prevention of PCM-induced hepatic toxicity in rats was associated with a decrease of oxidative stress in hepatic tissues. Keywords: Antihepatotoxicity; Chloroform extract of Argemone mexicana; Paracetamo